The UK government has sanctioned Russian-imposed leaders of breakaway regions in Ukraine.
Two leaders of the so-called Donetsk and Luhansk People's Republics have had their assets frozen.
Separatists in the eastern regions, which together make up the Donbas, set up unrecognised pro-Russian statelets in 2014 after the annexation of Crimea.
Foreign Secretary Liz Truss said the UK will keep imposing sanctions on those legitimising Russia's invasion.
On Tuesday, the government banned the so-called Donetsk People's Republic Prime Minister Vitaly Khotsenko and the so-called Luhansk People's Republic First Deputy Chairman Vladislav Kuznetsov from coming to the UK.
The UK Foreign Office said the pair supported Putin's plans to illegally annex more of Ukraine and were sent to the region to implement Russia's policies, where they have used sham referendums to falsely legitimise Russia's occupation.
Twenty-nine regional governors from across Russia have also been sanctioned. The UK says the governors had been directed by the Kremlin to transfer funds to the so-called Donetsk and Luhansk People's Republic to facilitate the occupation.
Foreign Secretary Liz Truss said: "We will not keep quiet and watch Kremlin-appointed state actors supress the people of Ukraine or the freedoms of their own people.
"We will continue to impose harsh sanctions on those who are trying to legitimise Putin's illegal invasion until Ukraine prevails."
Two nephews of major Russian oligarch Alisher Usmanov are now also subject to asset freezes.
Mr Usmanov was sanctioned in March for his association with Russian President Vladimir Putin. The oligarch is one of the wealthiest men in Russia, having made billions of dollars through his mining and telecom interests.
His nephews Sanjar Ismailov and brother Sarvar, previously a director at Everton FC, are believed to own homes in the London areas of Highgate and Hampstead Heath.
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